This trial was carried out in a commercial beef farm in Italy with a housing capacity for 600 Charolaise bulls. Animals are imported from France and arrive with an average live weight of around 415 kg after being transported for a period of 10 to 12 hours. Imported bulls aged from 12 to 15 months old. During the first 24 hours after arrival, all bulls are fed good quality hay and straw. From day 2-7, they receive a special adaptation diet consisting of hay, corn (silage and mashed), beet pulp, wheat straw and bran, soybean meal and complemented with hay, straw and a supplement for pulmonary support.
A total of 336 animals were included in the trial, distributed over six different batches. Within each batch, two groups of 14 animals were treated with the oral electrolyte solution containing potassium and glycerol through their drinking water for the first three days after arrival, whereas two control groups of 14 animals each were getting water only. Access to water in both groups was ad libitum. Animals were weighed and inspected upon arrival and groups of animals were randomly allocated to one of the four pens for the experimental period of 7 days.
Water and feed intake were recorded daily for the entire experimental period by measuring respectively replenishment of water levels and leftover feed. Clinical signs of respiratory and gastrointestinal disease (nasal/ocular discharge, ear position, temperature, cough, lameness and feces consistency), rumination activity and body condition scores were recorded at arrival and daily during the first 7 days after arrival.